FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10132289
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA127500.12583533691153104No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG120340.11876881916810703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106900.043.5396271
GGTATCA43700.039.479921
ATCAACG160150.028.708993
TCAACGC162050.028.3925444
CAACGCA164100.027.9912595
TATCAAC169800.027.3424282
AAAGTCG30350.027.04519359
AACGCAG176000.026.1571886
ACGAAAG31150.025.90736256
ATTAATC32200.025.71089645
AGGTTCG32450.025.39923967
GAACGAA31950.025.25878754
AGTCGGA34250.023.96559361
AAGTCGG35650.023.1211960
AGAACGA35200.022.9264353
CTAGGAC39800.022.4456063
CGCAATA14500.022.36996736
TCTAGCG14500.022.35749228
CTAGCGG15200.021.55678429
AATACGA15800.021.18532639
Produced by FastQC (version 0.11.5)