FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647266
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142900.14812486770863373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.046.9397241
GTATCAA156800.042.496321
TATCAAC218250.030.389852
ATCAACG218750.030.287913
TCAACGC221800.029.8398534
CAACGCA224350.029.5135
AACGCAG236200.028.1050626
ACGCAGA280750.023.6084427
CGCAGAG290400.022.8474468
AGAGTAC303350.021.87254511
GAGTACT134400.021.7843412
AAAGTCG15800.021.39115559
CAGAGTA318400.020.99009710
GCAGAGT318400.020.9574959
TCTAGCG8900.020.53777128
CTAGCGG9550.019.50286529
GTACTTT151150.019.4392114
AGTACTT152600.019.41229413
TACTTTT154900.019.21360615
CGCAATA9200.019.12873636
Produced by FastQC (version 0.11.5)