FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060196
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160530.15956945570444156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.048.2991681
GTATCAA198750.044.3107951
ATCAACG273450.032.290213
TCAACGC276200.031.9677544
TATCAAC277350.031.9606722
CAACGCA282550.031.2335385
AACGCAG293650.030.0644996
ACGCAGA348450.025.3759067
CGCAGAG359800.024.5562488
AGAGTAC370900.023.80298811
CAGAGTA386800.022.94020710
GAGTACT150900.022.85372412
GCAGAGT388850.022.8458759
AGTACTT163050.021.12947513
AGTACAT255850.021.02728713
GTACTTT167600.020.63846614
ACTTTTT171400.020.28170816
TACTTTT173200.020.19029815
GAGTACA271850.019.85323112
CGTCGTA20500.019.3458610
Produced by FastQC (version 0.11.5)