FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10009597
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.045.4035071
GTATCAA142900.044.52571
ATCAACG200500.031.7917583
TATCAAC201400.031.70172
TCAACGC202500.031.4761924
CAACGCA204200.031.1927365
AACGCAG214750.029.7565386
ACGCAGA258150.024.7673747
CGCAGAG272350.023.4886938
AGAGTAC275500.023.24528111
GAGTACT99750.022.6794912
AATTACG3850.022.39493656
CAGAGTA289350.022.20413610
CGCAATA7750.021.81775736
CCGCTTA7350.021.5844556
TATCGTC16850.021.28552863
GCAGAGT302000.021.2739549
CTAGGAC34500.021.0955243
AATCCGG21350.020.99868819
TCGACTA8400.020.94229930
Produced by FastQC (version 0.11.5)