FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10592116
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA179910.1698527470809421No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG173350.16365946143339066No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA140940.13306123158016775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109650.042.7840461
GGTATCA46550.040.056151
ATCAACG161650.028.7850363
TCAACGC163850.028.3764214
TCTAGCG15300.028.17693728
CAACGCA167500.027.7538765
ATTAATC41350.027.69669745
CTAGCGG15500.027.5920329
ACGAAAG42000.027.09752356
CGCAATA15550.026.85210436
AGGTTCG42250.026.77329467
AAAGTCG42550.026.66671459
TATCAAC176050.026.647472
AACGCAG179150.025.9485686
GAACGAA43550.025.8160854
AATACGA16200.025.7759239
AGTCGGA44850.025.37596761
AGAACGA44950.025.08838853
CTAGGAC43450.024.2125553
CGCGAAT6100.023.18084128
Produced by FastQC (version 0.11.5)