FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10592116
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA152730.14419215197416646No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG147490.13924507624350035No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA111850.10559740848759586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89800.042.498191
GGTATCA38650.038.394241
ATCAACG135100.028.2129823
CGCAATA15350.028.08974536
TCTAGCG16100.027.2090728
TCAACGC140850.027.183164
CAACGCA141750.027.108295
AATACGA16350.026.3709739
ATTAATC36750.026.09443945
ACGAAAG37250.025.74320456
TATCAAC148500.025.7151992
AAAGTCG38100.025.44024759
AACGCAG152250.025.2840636
GAACGAA38550.024.875254
CTAGCGG18050.024.46068829
AGGTTCG38600.024.395767
AGAACGA41000.023.47288553
AGTCGGA41650.023.27187261
ATACGAA19450.022.5225740
CAATACG19200.022.4570638
Produced by FastQC (version 0.11.5)