FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10540512
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217690.2065269694678968No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG130720.12401674605559958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.050.0644871
GTATCAA226450.047.674831
TATCAAC310700.034.7926032
ATCAACG322350.033.4591563
TCAACGC326150.033.04774
CAACGCA333550.032.350665
AACGCAG350900.030.8294356
ACGCAGA409300.026.4474627
CGCAGAG425250.025.4634788
AGAGTAC434750.024.8361411
CAGAGTA450500.024.03627810
GAGTACT195200.023.9385612
GCAGAGT452900.023.916529
TCGGATA6050.022.80238348
GTACTTT205600.022.66121314
TACTTTT213800.021.82443615
AGTACTT215750.021.70638713
ACTTTTT218650.021.5297116
AGTACAT284400.021.52322213
CTAGGAC57850.020.4505753
Produced by FastQC (version 0.11.5)