FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10417217
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141150.044.576771
GGTATCA53800.043.296251
TATCAAC199350.031.4561882
ATCAACG200550.031.0956763
TCAACGC201100.031.07764
CAACGCA204550.030.4815985
AACGCAG215650.028.9914846
ACGCAGA264550.023.6065647
CGCAGAG278550.022.4324748
CACGCGA5250.021.67619113
AGAGTAC290650.021.4751711
TATCGTC23800.021.4446763
CTAGGAC41950.021.0490383
GCAGAGT300800.020.807659
CAGAGTA305500.020.40861310
GAGTACT111950.019.89925412
CGTCGTA14050.019.88085210
ACGAAAG19800.019.85558356
ACCGTCG17950.019.4034548
AGTACAT207400.019.33745613
Produced by FastQC (version 0.11.5)