FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10417217
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96500.040.980911
GGTATCA43600.036.0250851
ATCAACG138050.028.6095143
TATCAAC140150.028.4533392
TCAACGC139900.028.3304944
CAACGCA142800.027.7071085
AACGCAG153500.025.8204296
TATATCG16450.021.59775260
ACGAAAG20250.021.12234956
ACGCAGA187650.020.992857
TACCGGT18100.020.5825447
CTAGGAC42400.020.4196953
ATTAATC21200.020.33863445
CGCAATA10100.020.15034336
AGTCGGA21700.020.02838761
AATCCGG21750.019.6655419
TATCGTC26850.019.65560763
CGCAGAG200650.019.5984488
AGGTTCG22100.019.3539167
GAATCCG23300.019.24548318
Produced by FastQC (version 0.11.5)