FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546302
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130990.12420467382784979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64400.045.761321
GTATCAA164800.044.0902331
TATCAAC233450.031.3828952
ATCAACG236700.030.8488123
TCAACGC241650.030.2019124
CAACGCA245850.029.7095055
AACGCAG259950.028.1903576
ACGCAGA314950.023.3113577
GAATCCG28850.022.59269518
AATCCGG29200.022.5582119
CGCAGAG326650.022.4657238
CTAGGAC45400.021.7283153
AGAGTAC338500.021.66937611
CAGAGTA350000.021.04586210
GCAGAGT351450.020.8804349
GAGTACT143100.020.67669712
TCTAGCG7950.020.3887728
CGCAATA8200.019.77794536
TACCGGT13100.019.743477
CACGCGA6050.019.38023813
Produced by FastQC (version 0.11.5)