FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546302
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105800.043.2723731
GGTATCA44650.039.1058961
TATCAAC151350.030.0857122
ATCAACG152150.029.9030083
TCAACGC156850.028.941134
CAACGCA159900.028.281235
AACGCAG168550.026.727516
ACGCAGA206600.021.487827
GAATCCG29300.021.42592618
AAAGTCG18350.021.24146359
CTAGGAC44150.020.5478823
TACCGGT14450.020.5293257
ACGAAAG19350.020.50072356
CGCAGAG217750.020.355848
AATCCGG30400.020.19707319
TCTAGCG9600.019.76258728
AGAGTAC223850.019.58503311
CGCAATA10150.019.37122236
AGTACAT225750.019.0377613
GAACGAA21750.019.03164954
Produced by FastQC (version 0.11.5)