FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26568164
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG419270.1578091734152198No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA410310.15443671606363163No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA393550.14812841414258057No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC349620.1315935869712337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319150.12012497363385742No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG313440.11797578485287881No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG305590.11502112076694497No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT295830.11134755115182216No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG293990.11065499294569245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136000.046.0361251
GTATCAA329050.040.7756841
ATCAACG449950.029.6603283
TCAACGC451750.029.5030254
CAACGCA454600.029.3241065
AACGCAG480050.027.8485136
ACGCAGA573450.023.2646037
CGCAGAG603250.022.1102988
TATCAAC634850.021.4071752
TCTAGCG121350.021.40690628
CTAGCGG123100.021.1866629
AGAGTAC636450.020.92983211
AAAGTCG144950.020.73141359
GCAGAGT651550.020.5453559
AGTCGGA145700.020.4829361
GTACATG749850.020.3929841
CAGAGTA659750.020.27431710
ACGAAAG147700.020.01624956
TACATGG738500.019.8322372
AGAACGA151050.019.68433253
Produced by FastQC (version 0.11.5)