FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485323
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184860.17630358168270066No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG112660.10744542633545959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.046.8378521
GTATCAA192500.045.585051
TATCAAC268750.032.7153552
ATCAACG273000.032.1042063
TCAACGC277450.031.6002164
CAACGCA282250.031.0342315
AACGCAG298450.029.3608136
ACGCAGA352950.024.8467887
CGCAGAG368550.023.8042018
AGAGTAC384650.022.73623511
CAGAGTA394550.022.25314510
GCAGAGT395650.022.1912759
GAGTACT166450.022.10627712
CTAGGAC49400.021.2259223
AATCCGG20100.020.93222419
GTACTTT178100.020.77672414
ACTTTTT184300.020.22766516
TACTTTT185800.020.0643615
AGTACAT255950.019.72546213
AGTACTT188650.019.63309113
Produced by FastQC (version 0.11.5)