FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485323
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118700.042.4953651
GGTATCA50000.040.311681
ATCAACG168000.029.8333423
TATCAAC169600.029.6970962
TCAACGC171750.029.3620154
CAACGCA175050.028.868295
AACGCAG185650.027.14536
ACGCAGA222400.022.4736187
CGCAGAG235650.021.1370968
AGAGTAC243950.020.41755111
CTAGGAC52800.020.3829143
CGGTCGA7550.020.10265420
AGTACAT239050.020.056413
CAGAGTA253450.019.67974710
GCAGAGT254650.019.5328279
CGCGAAT9650.018.9447128
CTCTAGG57700.018.7676961
CGTCGTA13800.018.74673710
GAGTACA258000.018.6236412
AATCCGG23900.018.18529319
Produced by FastQC (version 0.11.5)