FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11294863
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA220690.19538970946349682No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG199300.17645189676050077No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA132770.11754901321069589No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG115410.10217919420536575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117200.044.025081
GGTATCA48600.041.934711
ATTAATC38150.031.2843945
ACGAAAG39800.029.03239656
ATCAACG180350.028.6321323
AACGAAA40700.028.0516655
GAACGAA41100.027.8623354
TCAACGC185750.027.7988994
CAACGCA187850.027.4889815
TATCAAC188750.027.487662
AAAGTCG43100.027.45057759
CGCAATA18250.026.27171536
CATTAAT46450.026.13961844
AGGTTCG46350.025.30207367
AACGCAG208450.024.8384766
AGAACGA46450.024.80172553
AGTCGGA48800.024.45630861
TCTAGCG19950.024.20592528
AATACGA19400.024.18032839
CAATACG20500.023.21987238
Produced by FastQC (version 0.11.5)