FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10697294
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131200.044.280031
GGTATCA48850.042.0793381
TATCAAC190750.030.467522
ATCAACG192650.030.0226863
TCAACGC195050.029.6883584
CAACGCA198750.029.0983775
AACGCAG210300.027.4997396
CCGCTTA6900.026.49048856
TACCGGT22950.025.3946867
TATATCG22250.025.110260
AATCCGG25700.025.0928119
AGAACGA22050.024.08568653
CTAGCGG10250.023.88987529
AGGTTCG22250.023.7139767
CGCTTAC7800.023.43312657
GAATCCG27450.023.36745618
CGCAATA10800.023.32426636
TCTAGCG10500.023.31899828
ATTAATC23550.023.14446345
ACGAAAG22850.023.09236356
Produced by FastQC (version 0.11.5)