FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9841994
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113400.042.4220431
GGTATCA45750.041.053161
ATCAACG163150.029.8317623
TCAACGC165100.029.7726734
CAACGCA167850.029.3057375
TATCAAC170850.028.4694082
AACGCAG179250.027.2879876
ACGCAGA217800.022.3314977
CGCAGAG226550.021.4995568
AGTACAT228050.020.5556213
AGAGTAC234550.020.47200811
AAAGTCG25300.019.90541859
GCAGAGT246750.019.6138929
CGCAATA13050.019.56010636
GAATCCG20600.019.42369818
CAGAGTA247750.019.39558610
AGGTTCG25850.019.34816667
GAGTACA243800.019.27071612
CTAGCGG13200.019.07602729
CTAGGAC37400.018.722483
Produced by FastQC (version 0.11.5)