FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9932185
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA100910.10159899357492838No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG100770.10145803768254416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111300.041.962281
GGTATCA48150.038.856771
ATCAACG157850.029.8716873
TCAACGC162400.029.1834434
CAACGCA166400.028.462485
TATCAAC171500.027.3751222
AACGCAG175700.026.9364326
TCTAGCG16100.024.2112328
CGCAATA16000.023.06826636
CTAGCGG16650.022.3754429
AAAGTCG30500.022.27944859
AGGTTCG30450.022.20174667
ACGCAGA216150.021.8637777
ACGAAAG31400.021.64119156
AATACGA17100.021.38197339
GAACGAA32000.021.23531254
CGCAGAG225850.020.8943148
AGTCGGA33250.020.54011261
AGAGTAC229850.020.22972511
ATTAATC34700.020.08012845
Produced by FastQC (version 0.11.5)