FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10303375
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG124320.1206594926419741No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA123220.11959188130102999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112050.042.585551
GGTATCA49000.041.541011
ATCAACG162550.029.3259183
TCAACGC164900.028.9275054
CAACGCA167100.028.5628575
TATCAAC169900.028.301432
AACGCAG178800.026.770966
CGCGAAT6650.026.4496628
AAAGTCG28500.024.20194859
ACGAAAG28000.023.64853156
AGGTTCG28700.023.55239967
ATTAATC29800.022.45645945
GAACGAA29550.022.40830254
AGTCGGA30500.022.16263861
AGAACGA30300.022.08106853
ACGCAGA218700.021.902597
AATACGA11350.021.88907439
CGCAATA11300.021.67899136
CTAGCGG11300.021.67036229
AATCCGG22650.021.16406819
Produced by FastQC (version 0.11.5)