FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10303375
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG108600.10540235602411832No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA106450.10331566113045483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88900.040.4151271
GGTATCA41350.034.6546021
ATCAACG128800.027.878653
TCAACGC130050.027.7439794
CAACGCA133050.027.3522725
TATCAAC137500.026.1912212
ATTAATC27600.025.62089545
AACGCAG142600.025.3997756
AAAGTCG26850.024.79334859
ACGAAAG27850.024.52259356
AGGTTCG27900.024.47828767
GAACGAA28850.023.79214554
AGAACGA31050.022.66215753
AGTCGGA30850.022.58512761
AATCCGG23050.022.29729819
TCTAGCG12450.021.61047228
CTAGCGG12800.021.01945929
GAATCCG24800.020.86298818
AATACGA13250.020.30529439
ACGCAGA178600.020.2799977
Produced by FastQC (version 0.11.5)