FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110628
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA133620.13215796288816084No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG123130.1217827418830957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.045.3844951
GTATCAA132350.044.7977681
ATCAACG196250.030.1593
TCAACGC198050.029.8835664
TATCAAC200900.029.6508242
CAACGCA201700.029.3735035
AACGCAG215550.027.5497176
CGCAATA13150.027.29177536
TCTAGCG13850.025.64751228
ACGAAAG28050.025.08246256
CTAGCGG14750.024.0836529
AATCCGG19350.024.0592819
AAAGTCG29350.023.73670259
AATACGA15550.023.52477539
ATTAATC29800.023.26701545
AGGTTCG29900.023.18335367
GAACGAA31050.023.10264454
CGCGAAT4500.022.99145928
ACGGTCG5200.022.54831149
ACGCAGA265750.022.3455947
Produced by FastQC (version 0.11.5)