FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110628
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA117380.11609565696611525No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG109490.10829198740177168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94400.044.215311
GGTATCA39650.036.6460271
ATCAACG144200.029.0402833
TCAACGC147900.028.5923844
CAACGCA152350.027.8938985
TATCAAC153450.027.5389042
AACGCAG162250.025.979436
TATATCG15750.025.40495360
TACCGGT16900.024.9009697
CTAGGAC37600.022.4763433
AGGTTCG26650.022.39117867
ATTAATC27950.021.84498445
AATACGA14100.021.03835139
ACGAAAG27500.020.82108956
AAAGTCG28500.020.81740859
ACGCAGA202700.020.6759577
CGCAATA14550.020.62495236
AGTCGGA30300.020.37752261
TCTAGCG14900.020.37237428
CTAGTAC21250.020.1284433
Produced by FastQC (version 0.11.5)