FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10128759
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49700.040.542021
GTATCAA115550.040.315481
ATCAACG160550.029.4774913
TCAACGC162500.029.2508354
CAACGCA165500.028.8876345
AACGCAG171850.027.6997766
TATCAAC170150.027.6738032
ACGCAGA210050.022.4980377
AATCCGG23050.021.84885419
CGCAGAG219950.021.4227688
TACCGGT13750.021.0728517
GAATCCG24100.020.75359718
CGCAATA11000.020.6964536
AAAGTCG25050.020.6548359
AGGTTCG24500.020.55464267
AGAGTAC226750.020.53661511
TATATCG12650.020.45073360
CTAGCGG11150.020.41802229
ATACCGT26600.020.359396
AGTACAT221550.020.34864613
Produced by FastQC (version 0.11.5)