FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11735066
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93150.041.820141
GGTATCA41250.034.0483131
ATCAACG144350.027.0744933
TCAACGC150100.026.3123174
CAACGCA150750.026.2909495
TATCAAC152250.025.5583232
AACGCAG162050.024.2231856
ATTAATC30050.022.15412145
TCTAGCG15350.021.57251728
CGCAATA15400.021.50275236
ACGAAAG30700.021.23553756
AAAGTCG30750.020.75222259
TACCGGT15800.020.5214847
AATACGA16550.020.21659539
CTAGCGG16750.019.76944229
ACGCAGA199050.019.668527
AGGTTCG32750.019.58981767
AGTCGGA33150.019.35360561
GAATCCG22650.018.88429318
GAACGAA33650.018.86150454
Produced by FastQC (version 0.11.5)