FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10791966
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA189360.1754638589484066No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG177550.1645205331447486No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA131140.12151632056661409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113470.10514302954623839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70250.048.7637561
GTATCAA202900.045.3160361
ATCAACG287000.032.232713
TCAACGC294900.031.3794824
CAACGCA299750.030.9015485
TATCAAC304950.030.437932
TCTAGCG20250.029.29294428
AACGCAG321600.028.812646
CTAGCGG21550.027.68678529
AAAGTCG34250.027.18734459
GAACGAA36000.026.24881454
TACCGGT12000.025.5763267
AATACGA23250.024.7870539
ATTAATC38900.024.4740445
AGAACGA38700.024.4170553
ACGAAAG38850.024.23456456
AGTCGGA38850.024.14579461
ACGCAGA387900.023.8878637
CGCAATA24800.023.23710836
CGCAGAG399650.023.1853268
Produced by FastQC (version 0.11.5)