FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21899849
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG317060.1447772539436231No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA315630.14412428140486266No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA301660.13774524198774155No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG265470.12122001389142No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC262830.12001452612755457No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG257380.1175259244938173No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC249960.11413777327871073No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG247840.11316973007439457No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT240260.10970851899481132No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT234330.10700073776764396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA245300.044.455981
GGTATCA88100.041.6028631
ATCAACG337200.031.9674193
TCAACGC338750.031.8603524
CAACGCA340500.031.6342375
AACGCAG360250.029.9095256
ACGCAGA432850.024.8849647
CGCAGAG451200.023.88118
TATCAAC474350.023.1649322
GTACATG654550.023.0477451
TACATGG645700.022.4942632
AGAGTAC482550.022.36534711
GCAGAGT496350.021.7504759
CAGAGTA498250.021.7436810
TAACGGC26550.021.56675136
ACATGGG679150.021.1845663
AAAGTCG117100.021.18348759
AGTCGGA118450.020.76757661
TCTAGCG100950.020.53864328
AGTACAT433900.020.44531213
Produced by FastQC (version 0.11.5)