FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10162354
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87800.042.6675341
GGTATCA39800.031.5216331
ATCAACG129050.028.9740583
TCAACGC131050.028.5842364
CAACGCA135250.027.7995645
TATCAAC134650.027.6932852
AACGCAG146700.025.6062836
CGCGAAT4650.022.25391228
CTAGCGG10450.022.11538129
AATCCGG21000.021.02475219
GAACGAA21850.020.5228954
CGCAATA11100.020.50948536
TCTAGCG11150.020.41771528
ACGCAGA183350.020.4124667
GAATCCG22750.019.86222618
AAAGTCG22150.019.77705859
AATACGA11000.019.755539
ACGAAAG22700.019.75431456
CGCAGAG193550.019.283278
AGTCGGA23100.019.26216561
Produced by FastQC (version 0.11.5)