FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10445614
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG109250.10458935204766324No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA105850.10133439738439502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59350.044.509691
GTATCAA144250.043.4175721
ATCAACG201700.031.1745283
TCAACGC203650.030.8752844
CAACGCA206300.030.4578785
TATCAAC210150.029.9873892
AACGCAG219600.028.6597716
TCTAGCG11850.026.19261428
AGGTTCG29050.024.81124767
ACGAAAG29500.024.4334756
AAAGTCG29250.024.288759
CTAGCGG12950.023.96936229
CGCAATA13000.023.62340736
ACGCAGA267200.023.580257
GAACGAA30800.023.4024154
AGAACGA31100.023.17633253
ATTAATC31450.022.8157445
CGCAGAG278250.022.6432788
GAATCCG23450.021.91207718
AATACGA14550.021.81964739
Produced by FastQC (version 0.11.5)