FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10889959
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA135770.12467448224552545No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG128540.11803533879236826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118200.043.3327831
GGTATCA49100.041.170011
ATCAACG173250.029.6427383
TCAACGC176450.029.1241664
TATCAAC178750.028.7700392
CAACGCA179600.028.55265
ATTAATC27800.028.41602545
CGCAATA13150.027.8168836
CTAGCGG13800.027.742729
TCTAGCG13950.026.9463128
AACGCAG192450.026.7532756
AAAGTCG29900.026.1821759
AGGTTCG30450.025.93565267
ACGAAAG30850.025.71139556
AATACGA14700.024.88380639
GAACGAA31250.024.71991554
AGTCGGA33450.023.81578461
CAATACG16150.022.64965638
AGAACGA33850.022.41326153
CTAGGAC42100.022.1196653
Produced by FastQC (version 0.11.5)