FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10889959
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA116720.10718130343741422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94450.042.8953321
GGTATCA41750.037.175351
ATCAACG142050.028.3862553
TCAACGC146550.027.6781064
TATCAAC148500.027.2012712
CAACGCA149200.027.1645095
CGCAATA13450.026.15897236
AACGCAG160550.025.2442446
AATACGA13750.025.08673139
TCTAGCG14150.024.37711728
ATTAATC27400.024.29716145
AGGTTCG28350.023.1165767
ACGAAAG29600.022.72362956
GAACGAA29200.022.68052954
CTAGCGG15250.022.6185629
AAAGTCG29350.022.44729659
TACCGGT23800.022.3191977
CAATACG16100.021.85340338
ATACGAA15950.021.84265740
TATATCG22400.021.71200460
Produced by FastQC (version 0.11.5)