FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11176004
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108500.041.4972651
GGTATCA47350.039.7057881
ATCAACG152900.029.5789663
TCAACGC154600.029.2762874
CAACGCA158350.028.8877165
TATCAAC163550.027.5693422
AACGCAG168950.027.034576
AATCCGG26600.025.93786419
GAATCCG28850.023.6757218
TCTAGCG13200.022.21440528
CGCAATA12450.022.16697536
CTAGCGG13650.021.98752229
ACGCAGA210700.021.6613257
CGCAGAG222950.020.4711428
CTAGGAC41450.020.307343
ATCCGGC35350.019.517620
CGGATCC34600.019.34236340
AATACGA14500.019.27085139
AGTACAT230500.019.20135313
ACGAAAG25200.019.16494256
Produced by FastQC (version 0.11.5)