FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10772596
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA141630.13147248815420165No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG129730.12042594004267866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97750.044.6711771
GGTATCA40150.037.412531
ATCAACG150750.028.8305993
TCAACGC155050.028.1638664
CAACGCA157350.027.7091245
AAAGTCG30000.027.13470659
TATCAAC161750.026.9567432
ACGAAAG30700.026.85281856
ATTAATC31900.026.27678145
AGTCGGA32000.025.97750361
GAACGAA32000.025.86995554
AACGCAG170350.025.695676
AGGTTCG32450.025.5104967
AGAACGA34950.023.4890953
CGCAATA15500.022.9218736
TCTAGCG15000.022.76598428
AATACGA16100.021.85318439
GAATCCG24050.021.80103118
AATCCGG24200.021.66599819
AAGTCGG39000.020.96110260
Produced by FastQC (version 0.11.5)