FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10740155
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA116860.10880662336809852No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG111000.10335046375029038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.044.4423071
GTATCAA153950.043.927581
ATCAACG217700.031.0047573
TCAACGC220850.030.6086714
CAACGCA225050.030.0035765
TATCAAC234650.028.9568792
AACGCAG237700.028.4643366
TCTAGCG14500.025.92471528
CTAGCGG15500.024.92196329
CGCAATA16100.023.79015536
ACGCAGA291000.023.2863167
AATACGA16550.022.72735839
AAAGTCG32350.022.4941659
CGCAGAG303850.022.2900718
GAACGAA32000.021.98567254
AGAGTAC310350.021.76807211
ACGAAAG33550.021.17572656
AGGTTCG33450.021.0321467
CAGAGTA324300.021.0016510
AGTCGGA34550.020.96210161
Produced by FastQC (version 0.11.5)