FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10740155
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117350.043.860291
GGTATCA45650.036.3337251
ATCAACG168450.030.5934853
TCAACGC171000.030.1168144
CAACGCA174900.029.5641375
AACGCAG186100.027.6550086
TATCAAC188300.027.3513662
TCTAGCG16450.022.64639728
ACGCAGA229700.022.3755957
AAAGTCG29950.021.88265659
TACCGGT11050.021.5395267
CGCAGAG241850.021.2944858
CTAGCGG17900.021.0044229
AGGTTCG30900.020.87460967
GAACGAA31500.020.80550454
CGCAATA17600.020.77497736
ACGAAAG31800.020.60941756
AGAGTAC246400.020.60669711
ATTAATC32900.020.23572745
AGTACAT246400.020.00426913
Produced by FastQC (version 0.11.5)