FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10860280
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117810.1084778661323649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.045.4934771
GTATCAA154200.043.64161
TATCAAC220350.030.5227952
ATCAACG221350.030.18153
TCAACGC225850.029.5797334
CAACGCA228750.029.2017775
AACGCAG238500.028.0365266
ACGCAGA291100.022.9941887
CGCAGAG303500.022.088818
AGAGTAC308200.021.7521611
CTAGGAC43850.021.0792883
GAGTACT133050.020.57985912
CAGAGTA328450.020.48447210
AAAGTCG18800.020.36272459
GCAGAGT334600.020.118279
ACGAAAG19850.019.45934756
GTACTTT144150.019.13894814
TACTTTT146750.018.8233615
ACTTTTT150600.018.5023616
CGGTCGA7650.018.49324620
Produced by FastQC (version 0.11.5)