FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10860280
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100100.042.0441671
GGTATCA42600.037.3859181
ATCAACG142150.029.604323
TCAACGC145450.029.050564
CAACGCA147150.028.9731925
TATCAAC147000.028.7698272
AACGCAG157750.026.9609896
ACGCAGA192450.021.8846657
CGCAGAG205700.020.5087098
AGAGTAC206850.019.99391411
CTAGGAC42050.019.27723
AGTACAT209500.019.11500213
TACCGGT13300.018.9327357
CAGAGTA223900.018.68731110
AAAGTCG20650.018.37429859
TATATCG12800.018.32437160
CGCAATA9800.018.30313136
TCGACTA8550.018.15501230
GCAGAGT232550.017.992389
CGACTAT9050.017.91436631
Produced by FastQC (version 0.11.5)