FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004978389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116017
Sequences flagged as poor quality0
Sequence length25-322
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA1336811.52244929622383No Hit
GGATATTCTTAGTAGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATA1216710.487256177973919No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG96808.343604816535507No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG94888.178111828438936No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC87467.538550384857391No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC81977.065343872018756No Hit
TTACTCCCTTCTAGTAATTTGGGAATGCCTGGTTTATTTGGGACTCCAGC67375.806907608367739No Hit
CCCACTGTATTGAATTTTTACTCCCTTCTAGTAATTTGGGAATGCCTGGT35443.054724738615892No Hit
AGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATAAACCAGGCATTCCCAAATTACTAGAAGGGAGTAA23171.9971211115612366No Hit
TTACTCCCTTCTAGTAATTTGGGAATGCCTGGTTTATTTGGGACTCCAGCTCCAACCAGCTGGAAGGCGCT17581.5152951722592378No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC17421.5015040899178569No Hit
AACAACCAGAAATATTCCAACTATAATAGTAAGTATGAAACTTGTTTCTG12351.0644991682253464No Hit
AGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATAAACCAGGCATTCC11891.024849806493876No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT9910.8541851625192859No Hit
GAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAGGAACAAGAAGA9370.8076402596171252No Hit
GGATATTCTTAGTAGCGCCTTCCAGCTGGTTGGAGATGATTTTTGGATAC9130.7869536361050535No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA9110.785229750812381No Hit
GCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATGAATCAGCCACAA5490.4732065128386357No Hit
CTGACCTTTTGCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATT5310.4576915452045821No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTTGCAGCT5280.45510571726557314No Hit
GCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATTTCTTCCTCAT5140.44303852021686474No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCG3720.32064266443710837No Hit
CAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCGGGCTTTACG3500.3016799262177095No Hit
GACCCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTA3430.2956463276933553No Hit
ACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGT3100.26720222036425695No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT2860.24651559685218544No Hit
AGCGCCTTCCAGCTGGTTGGAGATGATTTTTGGATACCAGAAACAAGTTT2290.19738486601101565No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTG1640.1413585939991553No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC1510.1301533395967832No Hit
CCCACTGTATTGAATTTTTTACTCCCTTCTAGTAATTTGGGAATGCCTGG1430.12325779842609272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)