FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004978390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927403
Sequences flagged as poor quality0
Sequence length25-322
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA15980317.231236043014743No Hit
GGATATTCTTAGTAGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATA14252515.368184058063214No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC13192114.224776068224926No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG888599.581487228313906No Hit
TTACTCCCTTCTAGTAATTTGGGAATGCCTGGTTTATTTGGGACTCCAGC760378.198916760027734No Hit
CCCACTGTATTGAATTTTTACTCCCTTCTAGTAATTTGGGAATGCCTGGT467295.038694073665925No Hit
AGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATAAACCAGGCATTCCCAAATTACTAGAAGGGAGTAA399624.309022075624081No Hit
TTACTCCCTTCTAGTAATTTGGGAATGCCTGGTTTATTTGGGACTCCAGCTCCAACCAGCTGGAAGGCGCT281063.03061344420926No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG218022.3508658048334974No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC194882.10135183949157No Hit
AGCGCCTTCCAGCTGGTTGGAGCTGGAGTCCCAAATAAACCAGGCATTCC138461.4929863284893408No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC52320.5641560357255692No Hit
AACAACCAGAAATATTCCAACTATAATAGTAAGTATGAAACTTGTTTCTG30140.32499355727768836No Hit
ACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGT19410.20929412563901562No Hit
GGATATTCTTAGTAGCGCCTTCCAGCTGGTTGGAGATGATTTTTGGATAC17140.18481717225413333No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA11480.12378653077464706No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGATGGCAACGTTTTATGGTCCTTC11050.11914992727002176No Hit
GAAGGACCATAAAACGTTGCCATCCGGGCTTTACGCAAATAAGTAAGAAGGTCTCCTCCCTCCATCAGTTCC10500.1132193879036406No Hit
GCAATGGCGCAACCTTGGCTCACTGCAACCTCTACCTCTCAGGCTCAAGCGATTCTCCAGCCTCAGCCTC10170.1096610642838119No Hit
CCCACTGTATTGAATTTTACTCCCTTCTAGTAATTTGGGAATGCCTGGTT10140.10933758031837292No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT10110.10901409635293395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)