FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004978393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704825
Sequences flagged as poor quality0
Sequence length25-319
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG1259507.387855058437083No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC1233497.2352880794216405No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA1035066.071356297567199No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA951165.579223673984133No Hit
CCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTTAGGT934715.482732831815582No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT928105.443960523807429No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC921415.404718959423987No Hit
GGAGACTCAGGTGGAGTCATGCTTATATGGAGCAAAACTACTGTAGAGCC639713.752349947941871No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG555623.259102840467497No Hit
GCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATGAATCAGCCACAA512973.008930535392196No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC309481.8153182878008007No Hit
CTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGA257381.50971507339463No Hit
GGCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTAGATGTCTCGGGCCA191411.1227545349229393No Hit
CCAACAAGTGAAGGAGCTCTGCAGGGCCATCTTGGAGCCTGGGGATGTTC139630.8190283460179196No Hit
CCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGAGTCTCC109500.6422946636751573No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT94840.5563034329036705No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTG90340.5299077617937324No Hit
GTCTGCAGTGGACTTCTTTGCCCTGAAGAACTGCAGTGAAGGAACATCCC88580.5195841215374012No Hit
CTGCAGCTCCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACA84170.493716363849662No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGATTCATACAAT82320.482864810171132No Hit
GCAATGGCGCCATCACAGCTCGCTGCAGCCTC72570.425674189432933No Hit
GAGGCTGCAGCGAGCTGTGATGGCGCCATTGC72430.4248529907761794No Hit
GCCACCAAGATCTCATTGCTGTTTCTGGGC71520.41951519950728083No Hit
GGAGACTCAGTGTACCGCCGGAAGCACCAGGAGCTGCAAGCCATGCAGATGG64980.38115349082750427No Hit
GCCCAGAAACAGCAATGAGATCTTGGTGGC61520.36085815259630755No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATG57050.3346384526271025No Hit
CATACAATGGGTATGTCTTTTACCTCCTGAGAGATGGCATTTATAGAGCAG55390.32490138283988096No Hit
GGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAAG54200.3179211942574751No Hit
GAAGGACCATAAAACGTTGCCATCCGGGCTTTACGCAAATAAGTAAGAAGGTCTCCTCCCTCCATCAGTTCC51080.2996201956212515No Hit
CCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCGAGA49150.2882993855674336No Hit
GCAATGGCGCAACCTTGGCTCACTGCAACCTCTACCTCTCAGGCTCAAGCGATTCTCCAGCCTCAGCCTC48660.2854251902687959No Hit
GAAATGAGCAGGCACTCCTTGGAGCAAAAGCCCACTGC43990.25803234936137137No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGATGGCAACGTTTTATGGTCCTTC42390.24864722185561566No Hit
GCATGGTATGATCTCGGCTCATTGCAACCTCTGCCTCCCGGGTTCAAGCAAGTATCCTGCCTCAGCCTCTCC41330.24242957488305253No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC40150.23550804334755765No Hit
GCAGTGGGCTTTTGCTCCAAGGAGTGCCTGCTCATTTC34980.20518235009458452No Hit
CCTTACCCAAGATCTCATTGCTGTTTCTGGGC34630.20312935345270042No Hit
GCCCAGAAACAGCAATGAGATCTTGGACTTCC31240.18324461454988047No Hit
GTCTGCAGTGGACTTCTTTGCCTGAAGAACTGCAGTGAAGGAACATCCCC30740.18031176220433182No Hit
GGAAGTCCAAGATCTCATTGCTGTTTCTGGGC30640.17972519173522208No Hit
CCATCTGCATGGCTCCACCTGAGTCTCC30150.17685099643658442No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGA27870.1634771897408825No Hit
GACCCTGTACCTACATCAACCACGGCATCACCTTGACCCTGTACCTAC27610.1619521065211972No Hit
GCCCAGAAACAGCAATGAGATCTTGGGTAAGG27300.16013373806695702No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGTCAGCT24510.14376842197879547No Hit
GGCAGCATGGCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTAGATGTC23230.13626031997419089No Hit
CCTGGTGCTTCCGGCGGTACACTTTAGGTCCTTTCCCAGGTGTGGGCTCT22940.13455926561377266No Hit
AGAGGTTCCTGTCAAAGAAGGTGGCTCCGAGAAGTTTGT22770.13356209581628614No Hit
GACCCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTA21610.12675787837461322No Hit
CACCACAGAGAGGATCAGTGTGTGTGTGTGTCGTTGTTTG21340.12517413810801695No Hit
GTAGGTACAGGGTCAAGGTGATGCCGTGGTTGATGTAGGTACAGGGTC20820.12212397166864634No Hit
GACAGCCCCATCCATCTAGCTCACCCTGGAGCTGGGTTTGGGCACTGTAACATGTTGCAGGAGACCTGTCGG20310.11913246227618672No Hit
ACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGT20290.11901514818236476No Hit
CTGCAGCTCCATATAAGCATGACTCCACCTGAGTCTCC19330.11338407167891132No Hit
GATGGCTGGTGCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTT19080.111917645506137No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTTGCAGCT18980.11133107503702726No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA18430.10810493745692373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)