FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004978395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101363
Sequences flagged as poor quality0
Sequence length25-306
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA1200611.844558665390723No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT93859.25880252163018No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG93509.224273156871837No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC73057.206771701705751No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA72907.191973402523604No Hit
GCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATGAATCAGCCACAA44444.384242771030849No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG38913.8386788078490177No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC33283.2832493118790875No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA18761.8507739510472263No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTG18281.8034193936643548No Hit
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACATCTGTCCAGT15421.5212651559247459No Hit
CCAACTACACGTTACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGAT10371.0230557501257855No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAAATTGTGGCTGATTCATACA6600.6511251640144826No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC6470.6382999713899549No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT5000.49327663940491107No Hit
CTGCTCTATAAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTA4810.4745321271075244No Hit
CAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCGGGCTTTACG4440.438029655791561No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGATTCATACAAT4390.4330968893975119No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCG4120.4064599508696467No Hit
ACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGT3210.3166836024979529No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC3180.3137239426615234No Hit
CCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTGGTTG2920.2880735574124681No Hit
GCTCCCGTTAAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACAT2890.2851138975760386No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGA2620.2584769590481734No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTTGCAGCT1870.18448546313743674No Hit
TAATACCAAAAGTTACCAAAACTGCAGACAAGCATAAAGATGTCATCATC1700.16771405739766976No Hit
GAAGGACCATAAAACGTTGCCATCCGGGCTTTACGCAAATAAGTAAGAAGGTCTCCTCCCTCCATCAGTTCC1630.160808184446001No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAACTG1380.13614435247575546No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTTGCGTAAAGCCCGG1360.1341712459181358No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGATGGCAACGTTTTATGGTCCTTC1360.1341712459181358No Hit
GGAACTGATGGAGGGAGGAGATCTTCTTACTTATTTGCGTAAAGCCCGGA1350.13318469263932597No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAAGACATACCCATTGTA1320.13022503280289652No Hit
CTACAAGGTCAACCAAGGTGAATAAAGGACCATAAAACGTTGCCATCCGG1280.12627881968765722No Hit
GATGGCTGGTGCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTT1220.1203595000147983No Hit
GCCATCTCTCAGGAGGTAAAAAGACATACCCATTGTATGAATCAGCCACA1220.1203595000147983No Hit
ACTCCACCCCTCTATTAGTGCACCTCAAAAAAATTGTGGCTGATTCATAC1200.11838639345717866No Hit
GACCCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTA1190.11739984017836883No Hit
TCAACACCCAAATTAATACCAAAAGTTACCAAAACTGCAGACAAGCATAA1160.11444018034193937No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGAACCATAAAACGTTGCCATCCGG1110.10950741394789025No Hit
GGAACTGATGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGAT1020.10062843443860185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)