FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004978396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101102
Sequences flagged as poor quality0
Sequence length25-324
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA1085310.734703566695021No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG81278.038416648533165No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC74817.399457973136041No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC63126.243199936697592No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG62986.229352535063599No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT58555.79118118335938No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA55955.534015153013788No Hit
GCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATGAATCAGCCACAA30312.9979624537595697No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT20131.9910585349449073No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC12211.2076912425075665No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC9530.9426124112282646No Hit
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACATCTGTCCAGT9370.9267868093608436No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA9320.9218413087772744No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTG6960.6884136812328144No Hit
GATGGCTGGTGCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTT4790.4737789559059168No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAAATTGTGGCTGATTCATACA4050.40058554726909457No Hit
CAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCGGGCTTTACG3830.3788253447013907No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGATTCATACAAT3740.36992344365096635No Hit
CCAACTACACGTTACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGAT3680.36398884295068346No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTTGCAGCT3410.3372831397994105No Hit
AAATCAGGGAGTCAGATGGAGTGGACGCCGCAAGGTCCAAGTAGTCTCTC3170.31354473699827895No Hit
CTGCTCTATAAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTA2890.28584993373029216No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAAACGTTGCCATCCG2360.23342762754446003No Hit
GAAGGACCATAAAACGTTGCCATCCGGGCTTTACGCAAATAAGTAAGAAGGTCTCCTCCCTCCATCAGTTCC2340.2314494273110324No Hit
ACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGT2060.20375462404304562No Hit
GCCACCAAGATCTCATTGCTGTTTCTGGGC1950.19287452275919367No Hit
GCAATGGCGCAACCTTGGCTCACTGCAACCTCTACCTCTCAGGCTCAAGCGATTCTCCAGCCTCAGCCTC1950.19287452275919367No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGATGGCAACGTTTTATGGTCCTTC1870.18496172182548318No Hit
GCCCAGAAACAGCAATGAGATCTTGGTGGC1830.1810053213586279No Hit
GCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTA1780.17605982077505886No Hit
GACCCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTA1640.16221241914106546No Hit
GCAGTGGTGTGATCACAGCTCATTGCAGCTTTGACCTCCCTGGGCTTAGGTGATCCTCCCACCTCAGCCTC1540.15232141797392731No Hit
GCAATGGCGCCATCACAGCTCGCTGCAGCCTC1470.14539771715693062No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATG1430.14144131669007537No Hit
GCAGTGGTGTGATCACAGCTCATTGCAGCTTTGACCTCCCTGGGCTTGGGTGATCCTCCCACCTCAGCCTC1400.13847401633993395No Hit
CATACAATGGGTATGTCTTTTACCTCCTGAGAGATGGCATTTATAGAGCAG1370.1355067159897925No Hit
GAGGCTGCAGCGAGCTGTGATGGCGCCATTGC1360.13451761587307867No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGTCAGCT1200.11869201400565765No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTATAT1190.11770291388894384No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAATTGTGGCTGA1180.11671381377223002No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCTGGA1150.11374651342208858No Hit
GCTCCCTTTTCTTGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCA1120.11077921307194716No Hit
CCTTACCCAAGATCTCATTGCTGTTTCTGGGC1090.10781191272180571No Hit
GGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAAG1050.10385551225495045No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAAGACATACCCATTGTA1040.10286641213823663No Hit
CACTCCTGCCACGCTAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC1030.10187731202152281No Hit
GCTCCCGTTAAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACAT1020.100888211904809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)