FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004978399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662882
Sequences flagged as poor quality0
Sequence length25-328
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG12455518.789920377985826No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC12269318.50902573912099No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC375095.658473152084383No Hit
CGTCCTTCTTCTCCACCGGGTCTCCAGATGTGCTGTTAGTGTCTTCCAGG340955.143449362028234No Hit
CCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTTAGGT322264.861498728280448No Hit
TGCTGAGAGATCGGTAGCCAAGCTGGAAAAGACAATTGATGACCTGGAAG297484.4876765397159675No Hit
GGAGACTCAGGTGGAGTCATGCTTATATGGAGCAAAACTACTGTAGAGCC223683.3743562202624298No Hit
GAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAGGAACAAGAAGA140022.1122914787247202No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA134482.0287170265597796No Hit
GCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATTTCTTCCTCAT97281.4675311744775088No Hit
GGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGA84431.2736806852501652No Hit
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACATCTGTCCAGT70601.0650462676615144No Hit
CTGACCTTTTGCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATT69001.0409092417655026No Hit
ACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAA64360.970911866667069No Hit
CAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGGGCTTTACGC63030.9508479638910092No Hit
ACGGCAAGCAGCCCTGGTACCAGCTCTCCAACACGGAGGCAATCGACTGC62440.941947435591855No Hit
AAATCAGGGAGTCAGATGGAGTGGACGCCGCAAGGTCCAAGTAGTCTCTC62290.9396845894141038No Hit
GGTGGGCAGGCACGTGGCCGCTCCAACTCACGTCCCTGCGTGATGCAGTC61810.9324434816453003No Hit
CCAACTACACGTTACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGAT59520.8978973633316336No Hit
CTACAAGGTCAACCAAGGTGAGTAAAGGACCATAAAACGTTGCCATCCGG52240.7880738955047807No Hit
CTGCTCTATAAATGCCATCTCTCAGGAGGTAAAAGACATACCCATTGTAT48990.7390455616535069No Hit
GCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTA42690.644006022187961No Hit
GCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAAGACAATTGATGA42330.6385751913613584No Hit
AGACCTCTGGTGGGCAGGCACGTGGCCGCTCCAACTCACGTCCCTGCGTG39590.5972405345144385No Hit
GCCATCTCTCAGGAGGTAAAAGACATACCCATTGTATGAATCAGCCACAA31780.4794216768595315No Hit
CTGCAGCTCCATCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACA30730.4635817536152739No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGTCAGCT22500.33942692666266394No Hit
CTTCACCTACGGCAAGCAGCCCTGGTACCAGCTCTCCAACACGGAGGCAA22230.335353803542712No Hit
CTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGA20970.3163458956496028No Hit
GCAATGGTCTTTAGGTCAAGTTTACAACCAAGATTCACTGTGGATACAAT19140.28873917228103946No Hit
GCTCCCGTTAAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACAT17060.2573610386162243No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT15800.2383531307231151No Hit
GTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAG14890.2246251972447585No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTTGCAGCT14610.22040121771295645No Hit
GGCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTAGATGTCTCGGGCCA14000.211198976590102No Hit
CCTGCTGCTCGTCCAGCTTCCGAAGCCGGGTGTTGAGGTTTCCCCGAATA13650.20591900217534947No Hit
GCAATGGCGCAACCTTGGCTCACTGCAACCTCTACCTCTCAGGCTCAAGCGATTCTCCAGCCTCAGCCTC13010.19626419181694477No Hit
GACCCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTA12420.1873636635177905No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCTAGCTG10280.1550803913818749No Hit
CGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTG9220.1390896117257672No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC8810.1329044988399142No Hit
GGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAAG8720.13154679113326354No Hit
GCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCGCATCTGGAGTTCAGGA7300.1101251806505532No Hit
GTTTCGTCCTTCTTCTCCACCGGGTCTCCAGATGTGCTGTTAGTGTCTTC6790.10243150364619948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)