FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9416686
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425700.4520698683167305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG274000.011.1796811
ATATCGG13951.1202617E-49.080546145
TAGCGAA11503.221245E-58.091059
GCGTCGG31354.7293724E-108.081251145
AGAGCGG69950.06.255894145
AGCGTCG36402.1207492E-55.6946187145
AGATCGC33050.00112846675.226528145
CGTGTTT35455.10623E-45.1975327145
AGCTCGG62201.774606E-85.1839542145
CCGACCC62752.1880624E-85.1385174145
Produced by FastQC (version 0.11.5)