FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10884548
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG78350.020.9902655
CTATACT55400.019.7062444
GTATAGG36600.017.9852871
TACACTG89500.017.7497335
GTGTAGG61950.017.1819671
GTATAAG51000.017.1636811
TCTATAC50250.016.7121453
TATACCG7550.016.6845535
GTATAGA52400.016.3041881
GTCCTAG52000.015.8909261
TACACCG17250.015.8221155
GTATTAG49000.015.4347021
CTACACT62650.015.4151254
GTCTAGG50750.015.3164291
GTGTATA78950.015.16752051
CCTACAC57850.015.0004863
TACACAG108250.014.5460025
CTAGGAC49600.014.5325763
TAGGTCG10150.014.4791245
GTATATA126200.014.4272841
Produced by FastQC (version 0.11.5)