FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9378024
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67950.018.7518675
GTACTAG23700.017.3499871
GTATAGG33150.016.6088311
GTATAGA53950.016.5353741
TACACTG74700.016.4049515
CCTATAC32700.016.1820963
CTATACT47850.016.1513084
GTGTATA78800.015.7430661
TACACAG104000.015.5993135
TAGACTG52900.015.531355
TCTATAC46950.015.4229233
ACTGATC61950.015.3982518
GTGTAGG56700.015.3646421
GTCCTAG47100.014.7970311
ATAGGAC37650.014.6093563
GTATAAG43950.014.5889641
CTACACT55550.014.5392034
GTATTAG46050.014.3777021
CTAGACT43600.014.0527984
CCTACAC53650.014.01596453
Produced by FastQC (version 0.11.5)