FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11771453
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55000.050.12882145
ATACCGG22250.026.848019145
GTACTAA15250.019.8095341
GTTCTAG38450.018.2791731
GTATTAG17000.018.1329771
GTCCTAG37200.017.733271
CTATACT23450.017.6126464
GTATATA26050.017.5134641
GTCTTAG25700.017.0322971
GTATTAA29500.016.0922491
CTAGACT25300.015.837464
TCTAGAC27250.015.6090073
GTCTTAC42550.015.5035891
GTTCTAA29100.015.4659961
GGCTTAG34350.015.4354651
CTAACCT37000.015.3277634
TCTATAC25650.015.1406973
GGTCTAG33050.015.10989951
GTCCTAA25900.014.9964611
CTAGTAC12950.014.7565413
Produced by FastQC (version 0.11.5)