FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11771453
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120590.1024427485714805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA56250.045.83688145
GTATTAG17700.023.0216881
GCGAAAT21150.019.418749145
ATACCGG21200.019.37295145
GTTCTAG36750.018.9839951
GTACTAA16500.017.586541
GTGCTAA33300.017.0572931
GTCTTAA28250.016.6096941
GTTCTAA30400.016.4504551
GTCCTAG35000.016.4051931
GCCGTAT24550.015.799981145
TACACAA53700.015.7212305145
GGTCTAG31050.015.5095621
CGTATCA20700.015.4318075145
GTACATA27950.015.2417121
CTATACT22400.015.1544744
GTATAGG21750.015.0446861
CCTCTAG62400.015.0392261
GTACTAG13450.014.6890681
GTATTAA27100.014.580661
Produced by FastQC (version 0.11.5)