FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631941
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG116250.016.6979125
TATACTG95000.016.679985
CTATACT68000.016.5059644
GTATTAG60850.016.3915621
TAGACTG74000.015.5247765
CTACACT86650.015.0868144
CCTATAC46100.015.03797153
GTGTAGG90000.014.4586471
GTGTATA97550.014.3553091
GTATAGG44950.014.2543031
TATACAG99750.014.1647425
TCTATAC65550.014.0004833
TAATACT110400.013.9343834
GTATAAG62050.013.8390251
CTAGGAC64950.013.4182423
GTGTTAG75650.013.3593851
ATACTGT128100.013.3493426
TAGGACT74100.013.1869514
GTATAGA70450.013.1265621
CTATACC55500.013.0858094
Produced by FastQC (version 0.11.5)