FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631941
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA71000.016.5646481
GTATTAG63300.015.9701241
TATACTG90600.015.8857115
GTATAGG45900.015.4025171
GTGTATA99650.014.7196091
TACACTG113050.014.6582295
GTCCTAG67200.014.256711
CTATACT66150.014.1718224
CCTATAC43050.013.6476433
CCTACAC86250.013.5473693
TATACAG100400.013.4802685
TCTATAC64850.013.335283
GTGTAGG87000.013.2144961
CTAGGAC68600.013.0874663
GTGCTAG67950.013.0297461
CTAGTAC35000.013.0143323
GTCTTAG64500.012.9071821
GTATTAT107550.012.8397461
TTATACT118400.012.8245854
TAGGACT76300.012.8057314
Produced by FastQC (version 0.11.5)