FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705064
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146850.10715017456321253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG99400.019.585965
CTACACT76900.018.5529634
CTATACT69550.018.391534
GTATAGG45050.017.8163341
CCTATAC46350.017.5918083
TCTATAC64650.017.2874453
TACACTG111450.016.7115735
GTGTAGG80700.015.8783771
GTGTATA100150.015.8175731
CTTACAC76850.015.5491723
GTGCTAG70250.015.4341491
TCTACAC81050.015.0903213
TACACCG21500.015.0373345
CTATACC50500.015.0305884
CCTACAC74250.014.3896223
GTGTTAG80800.014.3774171
CTAGGAC61400.014.3101393
ATACGCG5000.00544394514.185126145
GCTATAC40600.014.0236553
ATACTGT132800.013.9729816
Produced by FastQC (version 0.11.5)